strem2 antibody  (Santa Cruz Biotechnology)

Journal: Biology

Article Title: sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway.

doi: 10.3390/biology13020087

Figure Lengend Snippet: Figure 2. sTREM2-induced cytokine expression in THP-1 cell model: treating THP-1 cells with 0.1 µg/mL or 1.0 µg/mL sTREM2 induced the expression of (A) TNF-α, (B) IL-1β, (C) TARC, and (D) IL-10. Data presented as Mean + SD.

Article Snippet: The cells were also treated with both 0.1 μg/mL sTREM2 + antibody + MCC950 (7.5 nM) (a specific NLRP3 inhibitor, Selleckchem.com (https://www.selleckchem.com/ (Accessed on 9 May 2022)).

Techniques: Expressing

Journal: Biology

Article Title: sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway.

doi: 10.3390/biology13020087

Figure Lengend Snippet: Figure 3. The effects of a TREM2 antibody (mAb) on sTREM2-induced cytokine expression: THP-1 cells were treated with 0.1 µg/mL sTREM2, mAb, or both sTREM2+mAb for 2, 4, 6, and 8 h, and cytokine expression of (A) TNF-α, (B) IL-1β, (C) TARC, and (D) IL-10 was assessed using qPCR. Data presented as Mean + SD.

Article Snippet: The cells were also treated with both 0.1 μg/mL sTREM2 + antibody + MCC950 (7.5 nM) (a specific NLRP3 inhibitor, Selleckchem.com (https://www.selleckchem.com/ (Accessed on 9 May 2022)).

Techniques: Expressing

Journal: Biology

Article Title: sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway.

doi: 10.3390/biology13020087

Figure Lengend Snippet: Figure 4. Inhibition of NLRP-3 inflammasome-mediated release of IL-1β induced by the complex of sTREM2+mAb. THP-1 cells were treated for 6 h with 0.1 µg/mL sTREM2, TREM2 antibody (mAb), or 0.1 µg/mL sTREM2+mAb (a monoclonal TREM2 antibody) and 0.1 µg/mL sTREM2+mAb with a selective NLRP3 inhibitor MCC950 (7.5 nM). Data presented as Mean + SD. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The cells were also treated with both 0.1 μg/mL sTREM2 + antibody + MCC950 (7.5 nM) (a specific NLRP3 inhibitor, Selleckchem.com (https://www.selleckchem.com/ (Accessed on 9 May 2022)).

Techniques: Inhibition

Journal: Biology

Article Title: sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway.

doi: 10.3390/biology13020087

Figure Lengend Snippet: Figure 5. The effects of sTREM2 on cytokine expression in THP-1-derived macrophages: (A). Cytokine expression in M0 macrophages following 2 h of sTREM2 treatment. (B). Cytokine expression in M1 macrophages following 2 h of sTREM2 treatment. (C). Cytokine expression in M2 macrophages following 2 h of sTREM2 treatment. (D). Cytokine expression in M0 macrophages following 8 h of sTREM2 treatment. (E). Cytokine expression in M1 macrophages following 8 h of sTREM2 treatment. (F). Cytokine expression in M2 macrophages following 8 h of sTREM2 treatment. Data presented as Mean + SD.

Article Snippet: The cells were also treated with both 0.1 μg/mL sTREM2 + antibody + MCC950 (7.5 nM) (a specific NLRP3 inhibitor, Selleckchem.com (https://www.selleckchem.com/ (Accessed on 9 May 2022)).

Techniques: Expressing, Derivative Assay

Journal: Biology

Article Title: sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway.

doi: 10.3390/biology13020087

Figure Lengend Snippet: Figure 6. The effects of Syk, NFκB, and JNK inhibitors on sTREM2-induced cytokine expression (A). The effect of TREM2 inhibitor piceatannol (15 µM) on cytokine expression; (B). The effect of MG-132 (100 nM) on cytokine expression; (C). The effect of JNK inhibitor SP600125 (90 nM) on cytokine. Data presented as Mean + SD.

Article Snippet: The cells were also treated with both 0.1 μg/mL sTREM2 + antibody + MCC950 (7.5 nM) (a specific NLRP3 inhibitor, Selleckchem.com (https://www.selleckchem.com/ (Accessed on 9 May 2022)).

Techniques: Expressing

Journal: Biology

Article Title: sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway.

doi: 10.3390/biology13020087

Figure Lengend Snippet: Figure 7. Levels of sTREM2 in the culture media of M0, M1, and M2 macrophages at 24 h and 48 h detected by ELISA.

Article Snippet: The cells were also treated with both 0.1 μg/mL sTREM2 + antibody + MCC950 (7.5 nM) (a specific NLRP3 inhibitor, Selleckchem.com (https://www.selleckchem.com/ (Accessed on 9 May 2022)).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Nature communications

Article Title: Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease.

doi: 10.1038/s41467-023-42505-x

Figure Lengend Snippet: Fig. 6 | Knockdown of TG2 abolishes the inhibitory effect of sTREM2 on tau phosphorylation. a p-Tau S202/T205 immunostaining and quantification of 7- month-old mice injected with sh-TG2 and sTREM2. Scale bar, 250 μm (mean ± s.e.m.; two-way ANOVA, n = 6 mice per group). b Western blots showing the

Article Snippet: To deplete sTREM2 from the CM, the TREM2 antibody (27599-1-AP, Proteintech) was added to the CM at a concentration of 10μg/ml and incubated for 2 h at 37 °C.

Techniques: Knockdown, Phospho-proteomics, Immunostaining, Injection, Western Blot